Publication: Distribution of chloroquine in normal, pronase-treated and malaria-infected red cells
Issued Date
1980-06-02
Resource Type
ISSN
00243205
Other identifier(s)
2-s2.0-0019314151
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Life Sciences. Vol.26, No.22 (1980), 1899-1903
Suggested Citation
Yongyuth Yuthavong Distribution of chloroquine in normal, pronase-treated and malaria-infected red cells. Life Sciences. Vol.26, No.22 (1980), 1899-1903. doi:10.1016/0024-3205(80)90619-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11187
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Title
Distribution of chloroquine in normal, pronase-treated and malaria-infected red cells
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Abstract
Pronase treatment of mouse red cells in the presence of chloroquine leads to greatly enhanced accumulation of the drug, which after freeze-thaw or hypotonic lysis is found to be located mainly in the membrane fraction. Much lower proportions of the drug are found in the membrane fraction prepared from Plasmodium berghei-infected red cells, which also have a high capacity for chloroquine accumulation. Pronase treatment of infected cells result only in a slight enhancement of total accumulation. The membrane-bound fraction of the drug is, however, increased while the fraction in the lysate is decreased. Membranes prepared from hypotonic lysis of normal or P. berghei-infected cells have similar capacities for chloroquine binding. These results show that the distribution of chloroquine in pronase-treated and malaria-infected cells are different and that pronase treatment of both normal and infected cells followed by lysis leads to availability of potential membrane binding sites. © 1980.