Publication: Direct determination of s-carboxymethyl-l-cysteine in syrups by reversed-phase high-performance liquid chromatography
5
Issued Date
2001-04-03
Resource Type
ISSN
07317085
Other identifier(s)
2-s2.0-0035079612
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.25, No.1 (2001), 165-170
Suggested Citation
Leena Suntornsuk Direct determination of s-carboxymethyl-l-cysteine in syrups by reversed-phase high-performance liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis. Vol.25, No.1 (2001), 165-170. doi:10.1016/S0731-7085(00)00479-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/26513
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Title
Direct determination of s-carboxymethyl-l-cysteine in syrups by reversed-phase high-performance liquid chromatography
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Abstract
A simple reversed-phase high-performance liquid chromatography method was developed for the determination of s-carboxymethyl-l-cysteine in syrup preparations. The experiments were performed without specific sample pre-treatment. The LC conditions used were acetonitrile-10 mM sodium dihydrogenphosphate buffer, pH 2.0 (1:99, v/v) on a C18Inersil column with a flow rate of 1.5 ml/min. Ultraviolet detection was carried out at 240 nm. The method showed excellent linearity (r2> 0.9998) over the concentration range tested (0.8-25.6 mg/ml) with good precision and accuracy (%R.S.D. 0.7%). Recoveries were good (> 99%) with a limit of detection and limit of quantitation of 0.1 and 0.8 mg/ml. Other compositions in the syrup vehicle did not interfere the analysis of s-carboxymethyl-l-cysteine. © 2001 Elsevier Science B.V.