Publication: A simple and efficient method for synthesis and extraction of ethyl ferulate from γ-oryzanol
Issued Date
2021-01-01
Resource Type
ISSN
13473352
13458957
13458957
Other identifier(s)
2-s2.0-85107942246
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Oleo Science. Vol.70, No.6 (2021), 757-767
Suggested Citation
Piraporn Sombutsuwan, Apiwat Jirattisakul, Akkaradech Nakornsadet, Saengchai Akepratumchai, Salisa Chumsantea, Siriluck Pojjanapornpun, Supathra Lilitchan, Kanit Krisnangkura, Kornkanok Aryusuk A simple and efficient method for synthesis and extraction of ethyl ferulate from γ-oryzanol. Journal of Oleo Science. Vol.70, No.6 (2021), 757-767. doi:10.5650/jos.ess20180 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76560
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A simple and efficient method for synthesis and extraction of ethyl ferulate from γ-oryzanol
Abstract
Ethyl ferulate (EF) is a ferulic acid (FA) derivative with high commercial value. It is not found naturally and is mostly synthesized from FA via esterification with ethanol. The present work aimed to synthesize the EF from γ-oryzanol, a natural antioxidant from rice bran oil via acid-catalyzed transethylation at refluxing temperature of ethanol. The reaction was optimized by central composite design (CCD) under response surface methodology. Based on the CCD, the optimum condition for the synthesis of EF from 0.50 g of γ-oryzanol was as follows: γ-oryzanol to ethanol ratio of 0.50:2 (g/mL), 12.30% (v/v) H2SO4, and a reaction time of 9.37 h; these conditions correspond to a maximum EF yield of 87.11%. Moreover, the optimized transethylation condition was further validated using 12.50 g of γ-oryzanol. At the end of the reaction time, distilled water was added as antisolvent to selectively crystallize the co-products, phytosterol and unreacted γ-oryzanol, by adjusting the ethanol concentration to 49.95% (v/v). The recovery yield of 83.60% with a purity of 98% of EF was achieved. In addition, the DPPH and ABTS assays showed similar antioxidant activities between the prepared and commercial EF.