Publication: Encapsidation defectiveness of herpes simplex virus type 2 during replication at acid pH condition
Issued Date
1994-01-01
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ISSN
0125877X
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2-s2.0-0028046710
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.12, No.1 (1994), 59-64
Suggested Citation
C. Yoosook, P. Bhattarakosol, P. Wilairat, S. Sriurairatna Encapsidation defectiveness of herpes simplex virus type 2 during replication at acid pH condition. Asian Pacific Journal of Allergy and Immunology. Vol.12, No.1 (1994), 59-64. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9722
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Title
Encapsidation defectiveness of herpes simplex virus type 2 during replication at acid pH condition
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Abstract
The maximal yield of herpes simplex virus type 2 (HSV-2) grown at pH 6.5 decreased 10 2 -10 3 fold compared to that recovered at pH 7.5. Electron microscopic observation of the infected cells maintained at these 2 pH conditions indicated that approximately equal amounts of immature virions were synthesized 6 hours after infection. However, at 18 hours post infection the majority of viruses present in the nucleus of infected cells maintained at pH 6.5 were empty or partially cored capsids with some particles enveloped and present in the cytoplasm, whereas at pH 7.5 mature virions already appeared at the cytoplasmic membrane. Analysis of viral polypeptides by radioimmunoprecipitation indicated that the synthesis of p40, a family of polypeptides closely involved in viral DNA encapsidation, was significantly impaired in infected cells maintained at pH 6.5.