Publication: Adaptor protein 1A facilitates dengue virus replication
Issued Date
2015-06-19
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ISSN
19326203
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2-s2.0-84939143087
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Mahidol University
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SCOPUS
Bibliographic Citation
PLoS ONE. Vol.10, No.6 (2015)
Suggested Citation
Umpa Yasamut, Nopprarat Tongmuang, Pa Thai Yenchitsomanus, Mutita Junking, Sansanee Noisakran, Chunya Puttikhunt, Justin Jang Hann Chu, Thawornchai Limjindaporn Adaptor protein 1A facilitates dengue virus replication. PLoS ONE. Vol.10, No.6 (2015). doi:10.1371/journal.pone.0130065 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35141
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Title
Adaptor protein 1A facilitates dengue virus replication
Abstract
© 2015 Yasamut et al. Rearrangement of membrane structure induced by dengue virus (DENV) is essential for replication, and requires host cellular machinery. Adaptor protein complex (AP)-1 is a host component, which can be recruited to components required for membrane rearrangement. Therefore, dysfunction of AP-1 may affect membrane organization, thereby decreasing replication of virus in infected cells. In the present study, AP-1-dependent traffic inhibitor inhibited DENV protein expression and virion production. We further clarified the role of AP-1A in the life cycle of DENV by RNA interference. AP-1A was not involved in DENV entry into cells. However, it facilitated DENV RNA replication. Viral RNA level was reduced significantly in Huh7 cells transfected with AP-1A small interfering RNA (siRNA) compared with control siRNA. Transfection of naked DENV viral RNA into Huh7 cells transfected with AP-1A siRNA resulted in less viral RNA and virion production than transfection into Huh7 cells transfected with control siRNA. Huh7 cells transfected with AP-1A siRNA showed greater modification of membrane structures and fewer vesicular packets compared with cells transfected with control siRNA. Therefore, AP-1A may partly control DENV-induced rearrangement of membrane structures required for viral replication.