Publication: Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins
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Issued Date
2006-03-01
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ISSN
00029637
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2-s2.0-33645964991
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.74, No.3 (2006), 348-352
Suggested Citation
Narisara Anuntagool, Vanaporn Wuthiekanun, Nicholas J. White, Bart J. Currie, Rasana W. Sermswan, Surasakdi Wongratanacheewin, Suwimol Taweechaisupapong, Sansanee C. Chaiyaroj, Stitaya Sirisinha Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins. American Journal of Tropical Medicine and Hygiene. Vol.74, No.3 (2006), 348-352. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23356
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Title
Short report: Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins
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Abstract
Heterogeneous patterns were obtained for lipopolysaccharide (LPS) from 1,327 Burkholderia pseudomallei isolates by sodium dodecyl sulfate- polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. Two LPS serotypes (A and B) possessing different ladder profiles and a rough LPS without ladder appearances were identified. All three LPS types were antigenically distinct by immunoblotting. The predominant type A (97%) produced the lowest amount of biofilm. The two less common types (smooth type B and rough type) were found more in clinical than environmental isolates and more in Australian isolates than Thai isolates. These isolates were more often associated with relapse than with primary infection. Copyright © 2006 by The American Society of Tropical Medicine and Hygiene.