Publication: Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination
Issued Date
2016
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Virology Journal. Vol. 13, (2016), 142
Suggested Citation
Nattika Nantachit, Panya Sunintaboon, Sukathida Ubol Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination. Virology Journal. Vol. 13, (2016), 142. doi:10.1186/s12985-016-0598-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2744
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Title
Responses of primary human nasal epithelial cells to EDIII-DENV stimulation: the first step to intranasal dengue vaccination
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Abstract
Background: About half of the world’s population are living in the endemic area of dengue viruses implying that
a rapid-mass vaccination may be required. In addition, a major target of dengue vaccine are children, thus, a
needle-free administration is more attractive. These problems may be overcome by the alternative route of
vaccination such as topical, oral and intranasal vaccination. Here, we investigated the possibility to deliver a
dengue immunogen intranasally, a painless route of vaccination. The tested immunogen was the domain III of
dengue serotype-3 E protein (EDIII-D3) loaded into trimethyl chitosan nanoparticles (EDIII-D3 TMC NPs). The
primary human nasal epithelial cells, HNEpCs, were used as an in vitro model for nasal responses.
Results: At tested concentrations, EDIII-D3 TMC NPs not only exerted no detectable toxicity toward HNEpC
cultures but also efficiently delivered EDIII-D3 immunogens into HNEpCs. Moreover, HNEpCs quickly and strongly
produced proinflammatory cytokines (IL-1β, IL-6, TNF-α), type-I IFN, the growth factors (GM-CSF, IL-7), the chemokines
(MCP-1, MIP-1β, IL-8), Th1-related cytokines (IL-2, IL-12p70, IL-17, IFN-γ) and Th2-related cytokine (IL-4) in response to
EDIII-D3 TMC NPs treatment.
Conclusions: A potential mucosal delivery system for dengue immunogens was revealed and found to stimulate a
strong local innate antiviral response which possibly leading to a systemic adaptive immunity.