Label-free detection of meca-mediated methicillin-resistant staphylococcus aureus (MRSA) using surface plasmon resonance spectroscopy

Abstract

© Springer International Publishing Switzerland 2014. The DNA hybridization of methicillin-resistant staphylococcus aureus (MRSA) was investigated by using surface plasmon resonance (SPR). 3 dimensional (3D) surface, carboxymethyl dextran hydrogel, were used as the sensor surface. The surface was functionalized with streptavidin where the biotinylated DNA probe can be specifically bound. Loop-mediated isothermal amplification (LAMP) products of MRSA were hybridized with the biotinylated probe and the biding was followed by SPR. The real time monitoring of hybridization kinetics of unlabeled mecA Lamp amplicons product was successfully carried out. The optimal condition of the probe density was 0.1 μM for MRSA at 0.06 nM. Non-specific absorption was not observed.