Publication: Protective effects of lotus stamen extract on uv-b-irradiated skin cells activities
12
Issued Date
2020-03-01
Resource Type
ISSN
01253395
Other identifier(s)
2-s2.0-85084083699
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Songklanakarin Journal of Science and Technology. Vol.42, No.2 (2020), 383-390
Suggested Citation
Preeyawass Phimnuan, Racharat Rukchay, Nichanan Tiraravesit, Sombat Wanauppathamkul, Suppisak Chaturonratsamee, Prapadsorn Pittayanurak, Vichai Reutrakul, Jarupa Viyoch Protective effects of lotus stamen extract on uv-b-irradiated skin cells activities. Songklanakarin Journal of Science and Technology. Vol.42, No.2 (2020), 383-390. doi:10.14456/sjst-psu.2020.50 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/56364
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Protective effects of lotus stamen extract on uv-b-irradiated skin cells activities
Other Contributor(s)
Abstract
© (Publication Year), (publisher Name). All rights reserved. Traditionally, Nelumbo nucifera has been used as a tonic and stimulant. The aim of this study was to clarify a protective effect regarding UV-B induced inflammation and melanogenesis activities of the crude extract of the dried lotus stamen extract. The compounds of the extract were isolated by column chromatography. The anti-oxidant EC50 value of the crude extract was 0.0353 mg/mL while the EC50 values of the isolated compounds of kaempferol and kaempferol 3-O-β-D-glucopyranoside were 0.0030 and 0.0077 mg/mL, respectively, which were observed by DPPH assay. The tyrosinase inhibitory activity was tested by tyrosinase enzyme. The anti-tyrosinase IC50 value of the crude was 0.0192 mg/mL while the isolated kaempferol was 0.0496 mg/mL. To determine the anti-inflammatory activity, the molecular targets for the extract action were TNF-α and IL-6. The release of TNF-α and IL-6 was significantly reduced in UV-B-induced keratinocytes pretreated with the crude extract at concentrations from 25 to 100 μg/mL. Moreover, the crude extract was found to inhibit the melanin overproduction of B16F1 induced by α-MSH.