Publication: Systematic comparisons of artificial urine formulas for in vitro cellular study
Issued Date
2010-07-01
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ISSN
10960309
00032697
00032697
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2-s2.0-77952745454
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Mahidol University
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SCOPUS
Bibliographic Citation
Analytical Biochemistry. Vol.402, No.1 (2010), 110-112
Suggested Citation
Somchai Chutipongtanate, Visith Thongboonkerd Systematic comparisons of artificial urine formulas for in vitro cellular study. Analytical Biochemistry. Vol.402, No.1 (2010), 110-112. doi:10.1016/j.ab.2010.03.031 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28672
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Title
Systematic comparisons of artificial urine formulas for in vitro cellular study
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Abstract
Artificial urine (AU) is widely used for in vitro cellular study to simulate normal physiological environment of the kidney and urinary tract. However, some compositions of many of previously established AU formulas are out of their physiological ranges in normal human urine. Therefore, we established a new AU formula, named " AU-Siriraj," and then performed systematic comparisons of AU-Siriraj with other six previously established AU formulas, ultrafiltrated (UF) urine, and blank-control to determine their compatibility with MDCK cells. The data indicate that AU-Siriraj is the best, and AU-3 is the second best, AU formula for in vitro cellular study. © 2010 Elsevier Inc.