Publication: Lemongrass-incorporated tissue conditioner against Candida albicans culture
Issued Date
2014-01-01
Resource Type
ISSN
0973709X
2249782X
2249782X
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2-s2.0-84904794069
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Clinical and Diagnostic Research. Vol.8, No.7 (2014), 50-52
Suggested Citation
Pokpong Amornvit, Suwan Choonharuangdej, Theerathavaj Srithavaj Lemongrass-incorporated tissue conditioner against Candida albicans culture. Journal of Clinical and Diagnostic Research. Vol.8, No.7 (2014), 50-52. doi:10.7860/JCDR/2014/8378.4607 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33331
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Title
Lemongrass-incorporated tissue conditioner against Candida albicans culture
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Abstract
Background: Tissue conditioner is applied popularly with dental prosthesis during wound healing process but it becomes a reservoir of oral microbiota, especially Candida species after long-term usage. Several antifungal drugs have been mixed with this material to control fungal level. In this study, lemongrass essential oil was added into COE-COMFORT tissue conditioner before being determined for anti-Candida efficacy. Materials and Methods: Lemongrass (Cymbopogon citratus) essential oil was primarily determined for antifungal activity against C. albicans American type culture collection (ATCC) 10231 and MIC (minimum inhibitory concentration) value by agar disk diffusion and broth microdilution methods, respectively. COE-COMFORT tissue conditioner was prepared as recommended by the manufacturer after a fixed volume of the oil at its MIC or higher concentrations were mixed thoroughly in its liquid part. Antifungal efficacy of the tissue conditioner with/without herb was finally analyzed. Results: Lemongrass essential oil displayed potent antifungal activity against C. albicans ATCC 10231and its MIC value was 0.06% (v/v). Dissimilarly, the tissue conditioner containing the oil at MIC level did not cease the growth of the tested fungus. Both reference and clinical isolates of C. albicans were completely inhibited after exposed to the tissue conditioner containing at least 0.25% (v/v) of the oil (approximately 4-time MIC). The tissue conditioner without herb or with nystatin was employed as negative or positive control, respectively. Conclusion: COE-COMFORT tissue conditioner supplemented with lemongrass essential oil obviously demonstrated another desirable property as in vitro anti-Candida efficacy to minimize the risk of getting Candidal infection.