Publication: Conserved neutralizing epitopes of HIV type 1 CRF01_AE against primary isolates in long-term nonprogressors
Issued Date
2004-01-01
Resource Type
ISSN
08892229
Other identifier(s)
2-s2.0-2642519580
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Mahidol University
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SCOPUS
Bibliographic Citation
AIDS Research and Human Retroviruses. Vol.20, No.5 (2004), 531-542
Suggested Citation
Apichai Sreepian, Surangrat Srisurapanon, Nawin Horthongkham, Somsith Tunsupasawasdikul, Surapol Kaoriangudom, Srisin Khusmith, Ruengpung Sutthent Conserved neutralizing epitopes of HIV type 1 CRF01_AE against primary isolates in long-term nonprogressors. AIDS Research and Human Retroviruses. Vol.20, No.5 (2004), 531-542. doi:10.1089/088922204323087787 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21423
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Title
Conserved neutralizing epitopes of HIV type 1 CRF01_AE against primary isolates in long-term nonprogressors
Abstract
Linear conserved B cell epitopes in envelope glycoprotein of long-term nonprogressors (LTNPs) HIV-1 CRF01_AE were determined. The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesized as peptides represent C1, C2, C3, C5, V2, V3, and gp41 regions. After that, the neutralizing B cell epitopes were determined by neutralized competitive assay with pool sera of typical progresser and LTNP HIV-1 CRF01_AE patients against HIV-1 CRF01_AE 24 primary isolates (PI) and laboratory strains (TCLA). We found that the strength and breadth of neutralization were greater for sera from LTNPs compared with sera from typical progressors. Peptides C1E and C2E could inhibit primary isolates but not the TCLA strain in LTNP sera. The new B cell epitopes, which were located in the C1 and C2 regions of CRF01_AE against primary HIV-1 isolates, were identified in HIV-1 CRF01_AE LTNPs. This may be important in HIV-1 vaccine development and trial.