Publication:
Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent

Issued Date

1979-03-27

Resource Type

Article

ISSN

00052795

DOI

10.1016/0005-2795(79)90024-2

Other identifier(s)

2-s2.0-0018802443

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

BBA - Protein Structure. Vol.577, No.1 (1979), 221-225

Suggested Citation

Jisnuson Svasti, Napasri Talupphet Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent. BBA - Protein Structure. Vol.577, No.1 (1979), 221-225. doi:10.1016/0005-2795(79)90024-2 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13247

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Title

Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent

Author(s)

Jisnuson Svasti
 Napasri Talupphet

Other Contributor(s)

Mahidol University

Abstract

By use of the neutral alkylating agent iodo [ 14 C 1 ] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis. © 1979.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/13247

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Scopus 1969-1990