Publication: Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis
Issued Date
2016-11-01
Resource Type
ISSN
14230380
10104283
10104283
Other identifier(s)
2-s2.0-84988632754
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Mahidol University
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SCOPUS
Bibliographic Citation
Tumor Biology. Vol.37, No.11 (2016), 14949-14960
Suggested Citation
Tanwarat Sanvoranart, Aungkura Supokawej, Pakpoom Kheolamai, Yaowalak U-pratya, Niphon Poungvarin, Sith Sathornsumetee, Surapol Issaragrisil Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis. Tumor Biology. Vol.37, No.11 (2016), 14949-14960. doi:10.1007/s13277-016-5314-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42769
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Title
Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis
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Abstract
© 2016, International Society of Oncology and BioMarkers (ISOBM). Glioblastoma (GBM) is an aggressive malignant brain tumor that still lacks effective therapy. Glioblastoma stem cells (GBM-SCs) were identified to contribute to aggressive phenotypes and poor clinical outcomes for GBM. Netrin-1, an axon guidance molecule, has been found in several tumors in adults. However, the role of Netrin-1 in GBM-SCs remains largely unknown. In this study, CD133-positive U251 GBM cells were used as a putative GBM-SC population to identify the functions of Netrin-1. Using lentiviral transduction, Netrin-1 miR RNAi vectors were transduced into CD133-positive U251 cells. We demonstrated that cell proliferation and survival were decreased following targeted deletion of Netrin-1. Cell invasion was dramatically diminished in Netrin-1 knockdown GBM-SCs. Moreover, Netrin-1 knockdown GBM-SCs exhibited less proangiogenic activity. In conclusion, Netrin-1 may represent a therapeutic target in glioblastoma.