Publication: Population structure of leishmania tropica causing anthroponotic cutaneous leishmaniasis in southern Iran by PCR-RFLP of kinetoplastid DNA
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Issued Date
2018-01-01
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ISSN
23146141
23146133
23146133
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2-s2.0-85048226183
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Mahidol University
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SCOPUS
Bibliographic Citation
BioMed Research International. Vol.2018, (2018)
Suggested Citation
Mohammad Amin Ghatee, Hossein Mirhendi, Masoud Marashifard, Zahra Kanannejad, Walter R. Taylor, Iraj Sharifi Population structure of leishmania tropica causing anthroponotic cutaneous leishmaniasis in southern Iran by PCR-RFLP of kinetoplastid DNA. BioMed Research International. Vol.2018, (2018). doi:10.1155/2018/6049198 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45330
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Title
Population structure of leishmania tropica causing anthroponotic cutaneous leishmaniasis in southern Iran by PCR-RFLP of kinetoplastid DNA
Abstract
Copyright © 2018 Mohammad Amin Ghatee et al. Iran is one of the six countries with the most cutaneous leishmaniasis (CL) patients. Understanding better the genotypes of the parasite population in relation to geography and climate is critical to achieving better CL control. We aimed to characterise the population structure of Leishmania tropica, the cause of anthroponotic cutaneous leishmaniasis (ACL), from important foci in southeast (Bam and Kerman) and southwest (Shiraz) Iran. A total of 39 L. tropica isolates from ACL patients from southeast (Bam 14, Kerman 12) and southwest (Shiraz 13) Iran were analysed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of the kinetoplast DNA (kDNA) using restriction enzymes MspI (HpaII) and ClaI. 37 genotypes were identified among south Iran L. tropica isolates. The unweighted pair group method with arithmetic mean (UPGMA) tree obtained from the banding patterns of ClaI digested kDNA RFLP distinguished southeast from and southwest L. tropica isolates with some subclustering but the MspI derived tree showed greater discrimination with greater subclustering and divergence of the two foci of southeast region but with some overlapping. Although a monophyletic structure has been defined for southeast L. tropica, isolates from two foci of southeast Iran were partly discriminated in the current study.