Publication: Role of CD137 signaling in dengue virus-mediated apoptosis
Issued Date
2011-07-08
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-79960050971
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.410, No.3 (2011), 428-433
Suggested Citation
Amar Nagila, Janjuree Netsawang, Chatchawan Srisawat, Sansanee Noisakran, Atthapan Morchang, Umpa Yasamut, Chunya Puttikhunt, Watchara Kasinrerk, Prida Malasit, Pa thai Yenchitsomanus, Thawornchai Limjindaporn Role of CD137 signaling in dengue virus-mediated apoptosis. Biochemical and Biophysical Research Communications. Vol.410, No.3 (2011), 428-433. doi:10.1016/j.bbrc.2011.05.151 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11515
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Title
Role of CD137 signaling in dengue virus-mediated apoptosis
Abstract
Hepatic dysfunction is a well recognized feature of dengue virus (DENV) infection. However, molecular mechanisms of hepatic injury are still poorly understood. A complex interaction between DENV and the host immune response contributes to DENV-mediated tissue injury. DENV capsid protein (DENV C) physically interacts with the human death domain-associated protein Daxx. A double substitution mutation in DENV C (R85A/K86A) abrogates Daxx interaction, nuclear localization and apoptosis. Therefore we compared the expression of cell death genes between HepG2 cells expressing DENV C and DENV C (R85A/K86A) using a real-time PCR array. Expression of CD137, which is a membe r of the tumor necrosis factor receptor family, increased significantly in HepG2 cells expressing DENV C compared to HepG2 cells expressing DENV C (R85A/K86A). In addition, CD137-mediated apoptotic activity in HepG2 cells expressing DENV C was significantly increased by anti-CD137 antibody compared to that of HepG2 cells expressing DENV C (R85A/K86A). In DENV-infected HepG2 cells, CD137 mRNA and CD137 positive cells significantly increased and CD137-mediated apoptotic activity was increased by anti-CD137 antibody. This work is the first to demonstrate the contribution of CD137 signaling to DENV-mediated apoptosis. © 2011 Elsevier Inc.