Publication: Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs
Issued Date
2017-03-01
Resource Type
ISSN
16159314
16159306
16159306
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2-s2.0-85012913499
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Separation Science. Vol.40, No.5 (2017), 1184-1192
Suggested Citation
Pornpan Prapatpong, Brompoj Prutthiwanasan, Nantana Nuchtavorn, Sawanya Buranaphalin, Leena Suntornsuk Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs. Journal of Separation Science. Vol.40, No.5 (2017), 1184-1192. doi:10.1002/jssc.201601162 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42201
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Title
Brompheniramine as a novel probe for indirect UV detection and its application for the capillary electrophoresis of adamantane drugs
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Abstract
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Brompheniramine, an antihistamine drug, was employed as a novel UV probe for capillary electrophoresis with indirect UV detection of adamantane drugs (memantine, amantadine, and rimantadine). The probe possesses high molar absorptivity of 24 × 103L/mol cm at 6 mM, which enables the measurement of these nonchromophore analytes without derivatization. The simple background electrolyte (10 mM sodium dihydrogen phosphate (pH 5.0) containing 5 mM brompheniramine and 6 mM β-cyclodextrin) provided the separation of the analytes in a short time (7.5 min). Under these conditions, brompheniramine had similar mobility to that of the analyte ions resulting in symmetric peaks with minimal electrodispersion. The analytes displace the probe at a one-to-one ratio with transfer values close to unity. β-Cyclodextrin played a role in the resolution of the structurally similar adamantane derivatives. Method validation showed good linearity (r2 > 0.98), precision (%RSD ≤ 3.30), and accuracy (recoveries ranging from 98 to 109%). The proposed method was successfully applied to determine the adamantane content in pharmaceutical products.