Publication: Human papillomavirus's physical state and cyclin A1 promoter methylation in cervical cancer
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Issued Date
2011-06-01
Resource Type
ISSN
15251438
1048891X
1048891X
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2-s2.0-82955165171
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Gynecological Cancer. Vol.21, No.5 (2011), 902-906
Suggested Citation
Pattamawadee Yanatatsaneejit, Apiwat Mutirangura, Nakarin Kitkumthorn Human papillomavirus's physical state and cyclin A1 promoter methylation in cervical cancer. International Journal of Gynecological Cancer. Vol.21, No.5 (2011), 902-906. doi:10.1097/IGC.0b013e3182158683 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12488
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Title
Human papillomavirus's physical state and cyclin A1 promoter methylation in cervical cancer
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Abstract
Background: Cervical cancer is the second biggest cause of death among human female cancers. Human papillomavirus (HPV) is the main factor in this cancer, especially HPV types 16 and 18, which constitute the high-risk group. There are 2 physical states of HPV in host cells: integrated and episomal forms. Our previous study explored the very high degree of cyclin A1 (CCNA1) promoter methylation in invasive cervical cancer in which all cases were infected by HPV. Objective: From previous evidence, it seemed that HPV might affect CCNA1 promoter methylation. Therefore, both the quantity and physical state of HPV were investigated in this study for their effects on CCNA1 promoter methylation. Materials and Methods: To determine the correlation of HPV quantity and CCNA1 methylation, the proportion of HPV L1/HAT (histone acetyltransferase, which is a human housekeeping gene) and the percentage intensity of CCNA1 promoter methylation were observed. CCNA1 promoter methylation was detected by methylation-specific primer polymerase chain reaction. To investigate the physical state, the HPV E2 region was amplified. The effect of the physical state on CCNA1 methylation was observed. Results: No correlation was found between the quantity of HPV and CCNA1 promoter methylation. Interestingly, the physical state of HPV had the potential to affect methylation of this gene. The integrated form of HPV had a significantly higher impact on CCNA1 methylation than HPV in episomal form (P = 0.001; 95% confidence interval, 11.96Y38.44). Conclusion: We suggest that the integrated form of HPV might lead to CCNA1 promoter methylation in cervical cancer by some mechanisms. Copyright © 2011 by IGCS and ESGO.