Publication: The nanostructure of rod-like ascorbyl dipalmitate nanoparticles stabilized by a small amount of DSPE-PEG
Issued Date
2021-06-01
Resource Type
ISSN
18733476
03785173
03785173
Other identifier(s)
2-s2.0-85107390564
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
International Journal of Pharmaceutics. Vol.602, (2021)
Suggested Citation
Ziqiao Chen, Kenjirou Higashi, Ryuhei Shidara, Keisuke Ueda, Takeshi Morita, Waree Limwikrant, Keiji Yamamoto, Kunikazu Moribe The nanostructure of rod-like ascorbyl dipalmitate nanoparticles stabilized by a small amount of DSPE-PEG. International Journal of Pharmaceutics. Vol.602, (2021). doi:10.1016/j.ijpharm.2021.120599 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/78958
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The nanostructure of rod-like ascorbyl dipalmitate nanoparticles stabilized by a small amount of DSPE-PEG
Other Contributor(s)
Abstract
Previously, we reported the formation of 100–200 nm disk- and tube-like nanoparticles by hydration of L-ascorbyl 2,6-dipalmitate (ASC-DP) and distearoylphosphatidylethanolamine polyethylene glycol 2000 (DSPE-PEG) films prepared at an initial molar ratio of 2:1. This study investigated the feasibility of nanoparticle formation with higher ASC-DP loading. Although particle size distribution determined by dynamic light scattering showed a multimodal pattern including micro-sized particles at a molar ratio of 3:1, the mean particle size gradually decreased with a further increased molar ratio. Homogeneous ca. 240 nm nanoparticles with a unimodal size distribution were obtained at a molar ratio of 10:1. FE-TEM showed that the nanoparticles at a molar ratio of 10:1 were rod-shaped with a diameter of ca. 100 nm and a length of ca. 300 nm. After centrifugation, X-ray analysis of the nanoparticle precipitates showed that these rod-like nanoparticles were composed of a series of lamellar structures with 3.7 nm repeated units. The molar ratio of ASC-DP/DSPE-PEG in the nanoparticle precipitates determined by 1H NMR measurements was 68.8:1. The rod-like nanoparticles should be composed of a core–shell structure, where a small amount of DSPE-PEG covers the lamellar structure of ASC-DP. Further increase in the ASC-DP/DSPE-PEG molar ratio over 33:1 no longer provided nanoparticles. Hence, to prepare a stable ASC-DP nanoparticle suspension, it is necessary to prepare ASC-DP/DSPE-PEG films containing at least 3 mol% DSPE-PEG.