Publication: Pharmacogenetic markers of CYP2B6 associated with efavirenz plasma concentrations in HIV-1 infected Thai adults
Issued Date
2012-12-01
Resource Type
ISSN
13652125
03065251
03065251
Other identifier(s)
2-s2.0-84869010225
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Mahidol University
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SCOPUS
Bibliographic Citation
British Journal of Clinical Pharmacology. Vol.74, No.6 (2012), 1005-1012
Suggested Citation
Chonlaphat Sukasem, Tim R. Cressey, Pattamawan Prapaithong, Yardpiroon Tawon, Ekawat Pasomsub, Chutatip Srichunrusami, Thawinee Jantararoungtong, Marc Lallement, Wasun Chantratita Pharmacogenetic markers of CYP2B6 associated with efavirenz plasma concentrations in HIV-1 infected Thai adults. British Journal of Clinical Pharmacology. Vol.74, No.6 (2012), 1005-1012. doi:10.1111/j.1365-2125.2012.04288.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14485
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Title
Pharmacogenetic markers of CYP2B6 associated with efavirenz plasma concentrations in HIV-1 infected Thai adults
Abstract
Aims: To investigate the frequency of CYP2B6 polymorphisms and the influence of haplotype structure on plasma efavirenz concentrations in Thai adults with HIV-1 infection. Methods: Genotyping of nine single nucleotide polymorphisms (SNPs, c.64C > T, c.499C > G, c.516G > T, c.785A > G, c.1375A > G, c.1459C > T, g.3003T > C, g.18492C > T and g.21563C > T) of CYP2B6 were performed using real-time PCR-based allelic discrimination on blood samples from 52 HIV-infected adults who had received an efavirenz-based regimen. Plasma efavirenz concentrations were measured by high performance liquid chromatography. Results: The minor allele frequencies for c.64C > T, c.516G > T, c.785A > G, g.3003C > T, g.18492T > C and g.21563C > T were 0.087, 0.365, 0.413, 0.308 and 0.356, respectively. However, no variant alleles were identified for three SNPs (c.499 C > G, c.1375 A > G and c.1459 C > T). Efavirenz plasma concentrations were significantly associated with c.516G > T (P= 0.0095), c.785A > G (P= 0.0017), g.21563C > T (P= 0.0036) and g.18492C > T (P= 0.0011). The composite CYP2B6 of three SNPs (c.516G ≥ T, c.785A ≥ G and g.21563C ≥ T) genotypes were significantly associated with higher efavirenz concentrations. Conclusions: Our data indicate that the GAC-CYP2B6 haplotype is associated with higher plasma efavirenz concentrations in HIV-infected Thai adults. © 2012 The Authors. British Journal of Clinical Pharmacology © 2012 The British Pharmacological Society.