Publication: Genomic evidence supporting the clonal expansion of extensively drug-resistant tuberculosis bacteria belonging to a rare proto-Beijing genotype
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Issued Date
2020-01-01
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ISSN
22221751
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2-s2.0-85097561954
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Mahidol University
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SCOPUS
Bibliographic Citation
Emerging Microbes and Infections. Vol.9, No.1 (2020), 2632-2641
Suggested Citation
Prapaporn Srilohasin, Therdsak Prammananan, Kiatichai Faksri, Jody E. Phelan, Prapat Suriyaphol, Phalin Kamolwat, Saijai Smithtikarn, Areeya Disratthakit, Sanjib Mani Regmi, Manoon Leechawengwongs, Rick Twee-Hee Ong, Yik Ying Teo, Sissades Tongsima, Taane G. Clark, Angkana Chaiprasert Genomic evidence supporting the clonal expansion of extensively drug-resistant tuberculosis bacteria belonging to a rare proto-Beijing genotype. Emerging Microbes and Infections. Vol.9, No.1 (2020), 2632-2641. doi:10.1080/22221751.2020.1852891 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/60503
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Title
Genomic evidence supporting the clonal expansion of extensively drug-resistant tuberculosis bacteria belonging to a rare proto-Beijing genotype
Other Contributor(s)
London School of Hygiene & Tropical Medicine
Khon Kaen University
National University of Singapore
Thailand Ministry of Public Health
Thailand National Center for Genetic Engineering and Biotechnology
Faculty of Medicine, Siriraj Hospital, Mahidol University
Vichaiyut Hospital
Thailand National Science and Technology Development Agency
Gandaki Medical College Teaching Hospital
Siriraj Foundation
Khon Kaen University
National University of Singapore
Thailand Ministry of Public Health
Thailand National Center for Genetic Engineering and Biotechnology
Faculty of Medicine, Siriraj Hospital, Mahidol University
Vichaiyut Hospital
Thailand National Science and Technology Development Agency
Gandaki Medical College Teaching Hospital
Siriraj Foundation
Abstract
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. Tuberculosis disease (TB), caused by Mycobacterium tuberculosis, is a major public health issue in Thailand. The high prevalence of modern Beijing (Lineage 2.2.1) strains has been associated with multi- and extensively drug-resistant infections (MDR-, XDR-TB), complicating disease control. The impact of rarer proto-Beijing (L2.1) strains is less clear. In our study of thirty-seven L2.1 clinical isolates spanning thirteen years, we found a high prevalence of XDR-TB cases (32.4%). With ≤ 12 pairwise SNP distances, 43.2% of L2.1 patients belong to MDR-TB or XDR-TB transmission clusters suggesting a high level of clonal expansion across four Thai provinces. All XDR-TB (100%) were likely due to transmission rather than inadequate treatment. We found a 47 mutation signature and a partial deletion of the fadD14 gene in the circulating XDR-TB cluster, which can be used for surveillance of this rare and resilient M. tuberculosis strain-type that is causing increasing health burden. We also detected three novel deletion positions, a deletion of 1285 bp within desA3 (Rv3230c), large deletions in the plcB, plcA, and ppe38 gene which may play a role in the virulence, pathogenesis or evolution of the L2.1 strain-type.