Publication: Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and Drebrin
Issued Date
2013-10-18
Resource Type
ISSN
15292401
02706474
02706474
Other identifier(s)
2-s2.0-84885454518
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Neuroscience. Vol.33, No.42 (2013), 16471-16482
Suggested Citation
Elliott B. Merriam, Matthew Millette, Derek C. Lumbard, Witchuda Saengsawang, Thomas Fothergill, Xindao Hu, Lotfi Ferhat, Erik W. Dent Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and Drebrin. Journal of Neuroscience. Vol.33, No.42 (2013), 16471-16482. doi:10.1523/JNEUROSCI.0661-13.2013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32711
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Title
Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and Drebrin
Abstract
Dendritic spines are actin-rich compartments that protrude from the microtubule-rich dendritic shafts of principal neurons. Spines contain receptors and postsynaptic machinery for receiving the majority of glutamatergic inputs. Recent studies have shown that microtubules polymerize from dendritic shafts into spines and that signaling through synaptic NMDA receptors regulates this process. However, the mechanisms regulating microtubule dynamics in dendrites and spines remain unclear. Here we show that in hippocampal neurons from male and female mice, the majority of microtubules enter spines from highly localized sites at the base of spines. These entries occur in response to synapse-specific calcium transients that promote microtubule entry into active spines. We further document that spine calcium transients promote local actin polymerization, and that F-actin is both necessary and sufficient for microtubule entry. Finally, we show that drebrin, a protein known to mediate interactions between F-actin and microtubules, acts as a positive regulator of microtubule entry into spines. Together these results establish for the first time the essential mechanisms regulating microtubule entry into spines and contribute importantly to our understanding of the role of microtubules in synaptic function and plasticity. © 2013 the authors.