Publication: Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide)
Issued Date
2006-10-01
Resource Type
ISSN
01757598
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2-s2.0-33749021065
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied Microbiology and Biotechnology. Vol.72, No.4 (2006), 726-731
Suggested Citation
Amnat Jarerat, Yutaka Tokiwa, Hideo Tanaka Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide). Applied Microbiology and Biotechnology. Vol.72, No.4 (2006), 726-731. doi:10.1007/s00253-006-0343-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22969
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Title
Production of poly(L-lactide)-degrading enzyme by Amycolatopsis orientalis for biological recycling of poly(L-lactide)
Author(s)
Abstract
Efficient production of poly(l-lactide)(PLA)-degrading enzyme was achieved by addition of 0.1% (w/v) silk fibroin powder into a liquid culture medium of an actinomycete, Amycolatopsis orientalis, without other complex nitrogen sources, such as yeast extract and peptone. Scaled-up production of the enzyme in a 5-l jar fermenter showed the possibility of producing this enzyme on an industrial scale at low production cost. The extracellular PLA-degrading enzyme showed potent degrading activity, which is effective for biological recycling of PLA, i.e., 2,000 mg/l of PLA powder was completely degraded within 8 h at 40°C using 20 mg/l purified enzyme. An optically active l-lactic acid with 600 mg/l was obtained as degradation product of PLA without undesirable racemization. © 2006 Springer-Verlag.