Publication: Anti-inflammatory action of two novel peptides derived from peanut worms (: Sipunculus nudus) in lipopolysaccharide-induced RAW264.7 macrophages
Issued Date
2020-01-01
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ISSN
2042650X
20426496
20426496
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2-s2.0-85078683589
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Mahidol University
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SCOPUS
Bibliographic Citation
Food and Function. Vol.11, No.1 (2020), 552-560
Suggested Citation
Papassara Sangtanoo, Piroonporn Srimongkol, Tanatorn Saisavoey, Onrapak Reamtong, Aphichart Karnchanatat Anti-inflammatory action of two novel peptides derived from peanut worms (: Sipunculus nudus) in lipopolysaccharide-induced RAW264.7 macrophages. Food and Function. Vol.11, No.1 (2020), 552-560. doi:10.1039/c9fo02178g Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/53530
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Title
Anti-inflammatory action of two novel peptides derived from peanut worms (: Sipunculus nudus) in lipopolysaccharide-induced RAW264.7 macrophages
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Abstract
© The Royal Society of Chemistry 2020. Peanut worm (Sipunculus nudus Linn.) protein was hydrolyzed by three proteases, and NO scavenging activity of the protein hydrolysates was evaluated. The hydrolysate obtained using Alcalase® showed the highest NO scavenging activity. This hydrolysate was fractionated using 10-, 5-, and 3 kDa molecular weight cut-off membranes, and the lowest MW fraction (<3 kDa) exhibited the highest NO scavenging activity. The <3 kDa fraction was further purified by gel filtration and high-performance liquid chromatographies. The peptides in the HPLC fraction with the strongest anti-NO activity were identified by quadrupole-time-of-flight mass spectrometry as LSPLLAAH (821.48 Da) and TVNLAYY (843.42 Da). Both peptides and the corresponding pure synthetic peptides inhibited NO production by RAW 264.7 macrophages without cytotoxicity. Quantitative real-time RT-PCR analysis showed that peptides LSPLLAAH and TVNLAYY reduced expression of proinflammatory cytokine genes iNOS, IL-6, TNF-α, and COX-2 in RAW 264.7 macrophages, suggesting that these peptides are novel anti-inflammatory candidates.