Publication: Farnesyltransferase inhibitor attenuates methamphetamine toxicity-induced Ras proteins activation and cell death in neuroblastoma SH-SY5Y cells
Issued Date
2013-06-17
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ISSN
18727972
03043940
03043940
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2-s2.0-84878537129
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Mahidol University
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SCOPUS
Bibliographic Citation
Neuroscience Letters. Vol.545, (2013), 138-143
Suggested Citation
Nipawan Pirompul, Piyarat Govitrapong, Banthit Chetsawang Farnesyltransferase inhibitor attenuates methamphetamine toxicity-induced Ras proteins activation and cell death in neuroblastoma SH-SY5Y cells. Neuroscience Letters. Vol.545, (2013), 138-143. doi:10.1016/j.neulet.2013.04.034 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/32714
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Title
Farnesyltransferase inhibitor attenuates methamphetamine toxicity-induced Ras proteins activation and cell death in neuroblastoma SH-SY5Y cells
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Abstract
Several lines of evidence support that methamphetamine (METH) toxicity plays a pivotal role in neurodegenerative diseases. However, the molecular mechanisms underlying METH-induced neurotoxicity are still unclear. In addition, Ras modulated death signaling has been continually reported in several cell types. In this study, intracellular Ras-dependent death signaling cascade activation was proposed to contribute to METH-induced neuronal cell degeneration in dopaminergic SH-SY5Y cultured cells. Exposure to a toxic dose of METH significantly decreased cell viability, and tyrosine hydroxylase phosphorylation, but increased c-Jun phosphorylation and active, GTP-bound Ras in cultured SH-SY5Y cells. Farnesyltransferase inhibitor, FTI-277, an inhibitor of the enzyme catalyzed the farnesylation of Ras proteins was able to diminish the toxic effects of METH on induction in cell degeneration, activation in c-Jun-N-terminal kinase cascades, and Ras activation in SH-SY5Y cells. The results of this study show that activation in Ras signaling cascade may be implicated in the METH-induced death signaling pathway in neuroblastoma SH-SY5Y cells. © 2013 Elsevier Ireland Ltd.