Publication: Allele Frequency-Based Analyses Robustly Map Sequence Sites Under Balancing Selection in a Malaria Vaccine Candidate Antigen
Issued Date
2003-10-01
Resource Type
ISSN
00166731
Other identifier(s)
2-s2.0-0242349712
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Genetics. Vol.165, No.2 (2003), 555-561
Suggested Citation
Spencer D. Polley, Watcharee Chokejindachai, David J. Conway Allele Frequency-Based Analyses Robustly Map Sequence Sites Under Balancing Selection in a Malaria Vaccine Candidate Antigen. Genetics. Vol.165, No.2 (2003), 555-561. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20688
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Allele Frequency-Based Analyses Robustly Map Sequence Sites Under Balancing Selection in a Malaria Vaccine Candidate Antigen
Other Contributor(s)
Abstract
The Plasmodium falciparum apical membrane antigen 1 (AMA1) is a leading candidate for a malaria vaccine. Here, within-population analyses of alleles from 50 Thai P. falciparum isolates yield significant evidence for balancing selection on polymorphisms within the disulfide-bonded domains I and III of the surface accessible ectodomain of AMA1, a result very similar to that seen previously in a Nigerian population. Studying the frequency of nucleotide polymorphisms in both populations shows that the between-population component of variance (FST) is significantly lower in domains I and III compared to the intervening domain II and compared to 11 unlinked microsatellite loci. A nucleotide site-by-site analysis shows that sites with exceptionally high or low FST values cluster significantly into serial runs, with four runs of low values in domain I and one in domain III. These runs may map the sequences that are consistently under the strongest balancing selection from naturally acquired immune responses.