K-ras oncogene and p53 gene mutations in cholangiocarcinoma from Thai patients

Abstract

Paraffin embedded tisues from twenty Thai patients with intrahepatic cholangiocarcinomas were studied forK-ras gene mutations at codon 12,13 and 61 and for p53 gene mutations in exon 5 to 8 using polymerase chain reaction and thermal cycle sequencing. Results showed that point mutations at these regions in K-ras oncogene were not present in all the samples. One case harbored ap53 gene mutation in codon 282 in exon 8, CGG (arginine) to TGG (tryptophan), but the mutation was not found in other patient's tissues with similar histological features.