Publication: Heterologous growth phase- and temperature-dependent expression and H<inf>2</inf>O<inf>2</inf>toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae
Issued Date
1996-01-01
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00219193
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2-s2.0-0029971105
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Bacteriology. Vol.178, No.12 (1996), 3578-3584
Suggested Citation
Skorn Mongkolsuk, Suvit Loprasert, Paiboon Vattanaviboon, Chaninchon Chanvanichayachai, Sangpen Chamnongpol, Niwat Supsamran Heterologous growth phase- and temperature-dependent expression and H<inf>2</inf>O<inf>2</inf>toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae. Journal of Bacteriology. Vol.178, No.12 (1996), 3578-3584. doi:10.1128/jb.178.12.3578-3584.1996 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17566
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Title
Heterologous growth phase- and temperature-dependent expression and H<inf>2</inf>O<inf>2</inf>toxicity protection of a superoxide-inducible monofunctional catalase gene from Xanthomonas oryzae pv. oryzae
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Abstract
Catalase is an important protective enzyme against H2O2toxicity. Here, we report the characterization of a Xanthomonas oryzae pv. oryzae catalase gene (katX). The gene was localized and its nucleotide sequence was determined. The gene codes for a 77-kDa polypeptide. The deduced katX amino acid sequence shares regions of high identity with other monofunctional catalases in a range of organisms from bacteria to eukaryotes. The transcriptional regulation of katX was atypical of bacterial monofunctional kat genes. Northern (RNA) analysis showed that katX transcription was highly induced by treatments with low concentrations of menadione, a superoxide generator, and methyl methanesulfonate, a mutagen. It was only weakly induced by H2O2. Unlike in other bacteria, a high level of catalase in Xanthomonas spp. provided protection from the growth-inhibitory and killing effects of H2O2but not from those of organic peroxides and superoxide generators. Unexpectedly, heterologous expression of katX in Escherichia coli was both growth phase and temperature dependent. Catalase activity in E. coli kat mutants harboring katX on an expression vector was detectable only when the cells entered the stationary phase of growth and at 28°C. The patterns of transcription regulation, heterologous expression, and physiological function of katX are different from previously studied bacterial kat genes.