Co-culture of males with late premolt to early postmolt female giant freshwater prawns, Macrobrachium rosenbergii resulted in greater abundances of insulin-like androgenic gland hormone and gonad maturation in male prawns as a result of olfactory receptors

Abstract

© 2019 Elsevier B.V. Insulin-like androgenic gland hormone (IAG) controls development of primary and secondary male sex-characteristics in decapod crustaceans. In male giant freshwater prawns, Macrobrachium rosenbergii, the IAG concentration correlates with male reproductive status and aggressiveness. When female prawns are co-cultured with males this can result in male size variations while this variation does not occur when males are cultured in monosex conditions. It was hypothesized that pheromone-like factors from female prawns may affect the abundance of IAG mRNA and protein in co-cultured males which would affect the pattern of sexual maturation of these males. In the present study, late premolt to postmolt females co-cultured with males for 7 days had a greater abundance of MrIAG mRNA transcript in all male phenotypes as well as for the gonad-somatic indexes (GSI). The abundance of MrIAG mRNA gradually increased from days 1 to 7 and using Western blot procedures MrIAG protein also increased in a similar pattern. Furthermore, with use of BrdU labeling, there was an increased cell proliferation in the spermatogenic zone of testicular tubules and in the spermatic duct epithelium during the 1 to 7 day co-culture period when there were increases in MrIAG mRNA and protein. In contrast, these effects were negated if short lateral antennules of males were ablated. Thus, results of the present study provide evidence that there might be female-molting factors which function as important regulators of androgenic gland function and gonadal maturation that were perceived by males via their short lateral antennules which are the olfactory organs.