Publication: Single-molecule recognition force spectroscopy of transmembrane transporters on living cells
Issued Date
2011-09-01
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ISSN
17502799
17542189
17542189
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2-s2.0-80052399829
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Mahidol University
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SCOPUS
Bibliographic Citation
Nature Protocols. Vol.6, No.9 (2011), 1443-1452
Suggested Citation
Theeraporn Puntheeranurak, Isabel Neundlinger, Rolf K.H. Kinne, Peter Hinterdorfer Single-molecule recognition force spectroscopy of transmembrane transporters on living cells. Nature Protocols. Vol.6, No.9 (2011), 1443-1452. doi:10.1038/nprot.2011.370 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11477
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Title
Single-molecule recognition force spectroscopy of transmembrane transporters on living cells
Abstract
Atomic force microscopy (AFM) has proven to be a powerful tool in biological sciences. Its particular advantage over other high-resolution methods commonly used is that biomolecules can be investigated not only under physiological conditions but also while they perform their biological functions. Single-molecule force spectroscopy with AFM tip-modification techniques can provide insight into intermolecular forces between individual ligand-receptor pairs of biological systems. Here we present protocols for force spectroscopy of living cells, including cell sample preparation, tip chemistry, step-by-step AFM imaging, force spectroscopy and data analysis. We also delineate critical steps and describe limitations that we have experienced. The entire protocol can be completed in 12 h. The model studies discussed here demonstrate the power of AFM for studying transmembrane transporters at the single-molecule level. © 2011 Nature America, Inc. All rights reserved.