Publication: Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus
Issued Date
2008-03-17
Resource Type
ISSN
0145305X
Other identifier(s)
2-s2.0-41949127659
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Developmental and Comparative Immunology. Vol.32, No.9 (2008), 1063-1075
Suggested Citation
Nipaporn Kanthong, Nuanpan Khemnu, Siriporn Sriurairatana, Sa Nga Pattanakitsakul, Prida Malasit, Timothy W. Flegel Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus. Developmental and Comparative Immunology. Vol.32, No.9 (2008), 1063-1075. doi:10.1016/j.dci.2008.02.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18949
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus
Abstract
To study persistent viral co-infections in arthropods, we first produced stable, persistently infected C6/36 mosquito cell cultures by serial passage of exponentially growing whole cells infected with either a densovirus (AalDNV) or Dengue virus (DEN-2). We then obtained stable, persistent co-infections by reciprocal super-challenge and similar passaging. Persistently infected cultures did not differ from naïve-cell cultures in growth rate and cell morphology. Nor did they differ in high production of both viruses with high infection rates for naïve C6/36 cells. Immunocytochemistry revealed that 99-100% of the cells were coinfected but that super-infection order had some effect on antigen distribution for the two viruses. Our results combined with existing field information and previously published experimental work suggest that the capacity to support stable, viral co-infections may be a general phenomenon for arthropod cells, and that they may be achieved easily and rapidly by serial passaging of whole cultured cells. Such persistent infections would facilitate studies on interactions between co-infecting viruses. © 2008 Elsevier Ltd. All rights reserved.