Publication: Identification of pathogens causing invasive fungal rhinosinusitis in surgical biopsies using polymerase chain reaction
Issued Date
2020-01-01
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ISSN
17485460
00222151
00222151
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2-s2.0-85088535352
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Laryngology and Otology. (2020)
Suggested Citation
S. Chaturantabut, N. Kitkumtorn, A. Mutirangura, N. Praditphol, A. Chindamporn, P. S. Thorner, S. Keelawat Identification of pathogens causing invasive fungal rhinosinusitis in surgical biopsies using polymerase chain reaction. Journal of Laryngology and Otology. (2020). doi:10.1017/S0022215120001395 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/58300
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Title
Identification of pathogens causing invasive fungal rhinosinusitis in surgical biopsies using polymerase chain reaction
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Abstract
Copyright © The Author(s), 2020. Published by Cambridge University Press. BackgroundInvasive fungal rhinosinusitis is associated with high morbidity and mortality. Rapid pathogen identification is mandatory, but fresh tissue is not always available. A polymerase chain reaction method was designed in order to detect fungi in formalin-fixed paraffin-embedded samples. This was applied to a retrospective series of tissue biopsies from Thai patients with invasive fungal rhinosinusitis.MethodsTissue blocks from 64 cases yielded adequate DNA. Three sequential polymerase chain reaction were performed: ZP3 (housekeeping gene) and panfungal polymerase chain reactions, and a differentiating polymerase chain reaction based on the 5.8s ribosomal RNA and internal transcribed spacer 2 regions. The polymerase chain reaction products were then sequenced.ResultsPolymerase chain reaction identified a fungal pathogen in 20 of 64 cases (31 per cent). Aspergillus species was the most common cause of invasive fungal rhinosinusitis (nine cases). Other causes included candida (n = 4), cladosporium (n = 4), mucor (n = 1), alternaria (n = 1) and dendryphiella (n = 1) species.ConclusionPolymerase chain reaction can provide rapid identification of fungal pathogens in paraffin-embedded tissue, enabling prompt treatment of invasive fungal rhinosinusitis.