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Antimicrobial susceptibility testing of Leptospira spp. In the Lao People’s democratic Republic using disk diffusion

dc.contributor.authorJennifer Bossen_US
dc.contributor.authorDavid A.B. Danceen_US
dc.contributor.authorAnisone Chanthongthipen_US
dc.contributor.authorPaul N. Newtonen_US
dc.contributor.authorVanaporn Wuthiekanunen_US
dc.contributor.authorMatthew T. Robinsonen_US
dc.contributor.otherLondon School of Hygiene & Tropical Medicineen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherMahosot Hospitalen_US
dc.date.accessioned2020-01-27T09:05:06Z
dc.date.available2020-01-27T09:05:06Z
dc.date.issued2019-01-01en_US
dc.description.abstractCopyright © 2019 by The American Society of Tropical Medicine and Hygiene Leptospirosis is a global zoonotic disease caused by pathogenic bacteria of the Leptospira genus, which are fastidious, slow-growing organisms. Antimicrobial susceptibility data are limited; traditionally, the organisms have not been culturable on solid media. The recent development of Leptospira Vanaporn Wuthiekanun (LVW) agar, which facilitates rapid growth of Leptospira spp., provides the opportunity for antimicrobial susceptibility testing. Eighty-three Leptospira spp. clinical isolates originating from patients in Laos between 2006 and 2016 were tested against six antimicrobials (azithromycin, ceftriaxone, ciprofloxacin, doxycycline, gentamicin, and penicillin G) using disk diffusion on LVW agar. Quality control was undertaken using American Type Culture Collection (ATCC) reference strains with known susceptibilities on both standard media and LVW agar. All Leptospira spp. isolates produced large zones of inhibition around each of the six antimicrobials. All zones were greater than 25 mm: gentamicin produced the smallest zones (median 35 mm; interquartile range 30 mm–37 mm) and azithromycin produced the largest zones (median 85 mm; interquartile range 85 mm–85 mm). Zones produced by non-leptospiral ATCC reference strains on LVW agar were within 2 mm of accepted strain-specific quality control range on standard media. Antimicrobial activity on LVW agar appears to be similar to that on standard media. As there are no published susceptibility guidelines for the Leptospira genus, zone interpretation was subjective. Leptospira Vanaporn Wuthiekanun agar enabled antimicrobial susceptibility testing of multiple Leptospira isolates on solid media; the large zone sizes observed suggest that resistance has not emerged to these six antimicrobials in Lao Leptospira spp.en_US
dc.identifier.citationAmerican Journal of Tropical Medicine and Hygiene. Vol.100, No.5 (2019), 1073-1078en_US
dc.identifier.doi10.4269/ajtmh.18-0955en_US
dc.identifier.issn00029637en_US
dc.identifier.other2-s2.0-85065510194en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/51146
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065510194&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleAntimicrobial susceptibility testing of Leptospira spp. In the Lao People’s democratic Republic using disk diffusionen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065510194&origin=inwarden_US

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