Publication: Isolation of pseudomonas pseudomallei from soil in north-eastern Thailand
Issued Date
1995-01-01
Resource Type
ISSN
18783503
00359203
00359203
Other identifier(s)
2-s2.0-0028926865
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.89, No.1 (1995), 41-43
Suggested Citation
Vanaporn Wuthiekanun, Michael D. Smith, David A.B. Dance, Nicholas J. White Isolation of pseudomonas pseudomallei from soil in north-eastern Thailand. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.89, No.1 (1995), 41-43. doi:10.1016/0035-9203(95)90651-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17330
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Title
Isolation of pseudomonas pseudomallei from soil in north-eastern Thailand
Abstract
In order to optimize the recovery from soil of Pseudomonas pseudomallei, the cause of melioidosis, 3 selective broths were compared. A basal salt solution containing l-threonine (TBSS) performed significantly better than trypticase soy broth containing crystal violet and colistin 50 mg/L (CVC50), both in isolation rate and suppression of overgrowth of other organisms, but the addition of colistin to TBSS gave the best results overall. In a survey in north-eastern Thailand, P. pseudomallei was recovered from 114 (68%) of the 167 sites tested. A detailed study of a single rice farm showed that the isolation rate increased with depth of soil sample, and P. pseudomallei could still be isolated during the dry season, although only from moist soil in areas where other crops were cultivated and around the water source. © 1995 Oxford University Press.