Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.2 (2010), 280-284
Suggested Citation
Parima Boontanom, Suradej Siripattanapipong, Mathirut Mungthin, Peerapan Tan-Ariya, Saovanee Leelayoova Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.41, No.2 (2010), 280-284. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29744
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Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen
A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).
