Publication: Enhanced chondrogenesis through specific growth factors in a buffalo embryonic stem cell model
Issued Date
2013-08-19
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ISSN
10958355
10656995
10656995
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2-s2.0-84885863159
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Mahidol University
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SCOPUS
Bibliographic Citation
Cell Biology International. Vol.37, No.11 (2013), 1246-1258
Suggested Citation
Hathaitip Sritanaudomchai, Yindee Kitiyanant, Pirut Tong-ngam, Chareonsri Thonabulsombat, Kenneth L. White, Thanit Kusamran Enhanced chondrogenesis through specific growth factors in a buffalo embryonic stem cell model. Cell Biology International. Vol.37, No.11 (2013), 1246-1258. doi:10.1002/cbin.10153 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31242
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Title
Enhanced chondrogenesis through specific growth factors in a buffalo embryonic stem cell model
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Abstract
Chondrogenic differentiation of embryonic stem cells (ESCs) via embryoid bodies (EBs) is an established model to investigate chondrogenesis signaling pathways and molecular mechanisms in vitro. Our aim has been to improve upon the number of differentiated cells needed for the in vitro development of functional cartilage. Chondrogenic differentiation of buffalo ESCs was modulated by bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 10 (FGF-10), transforming growth factor-beta1 (TGF-β1) individually and their combination. ESCs differentiation into chondrocytes was characterized by the appearance of Alcian blue-stained nodules and the expression of cartilage-associated genes (RT-PCR) and protein (immunocytochemistry). BMP-2 or FGF-10 treatment enhanced chondrogenic differentiation, whereas TGF-β1 treatment inhibited buffalo ESC-derived chondrogenesis. The combination of BMP-2 and FGF-10 was the most effective treatment. This treatment resulted in a higher number of Alcian blue-positive nodules by 15.2-fold, expression of the mesenchymal cell marker scleraxis gene by 3.25-fold, and the cartilage matrix protein collagen II gene and protein 1.9- and 7-fold, respectively, compared to the untreated control group. Chondrogenesis was also recapitulated from mesenchymal and chondrogenic progenitor cells, resulting in the establishment of mature chondrocytes. Thus, buffalo ESCs can be successfully triggered in vitro to differentiate into chondrocyte-like cells by specific growth factors, which may provide a novel in vitro model for further investigation of the regulatory mechanism(s) involved. © 2013 International Federation for Cell Biology.