Publication:
Giant catfish Pangasianodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction

Issued Date

1994-11-18

Resource Type

Article

ISSN

03781119

DOI

10.1016/0378-1119(94)90160-0

Other identifier(s)

2-s2.0-0027984046

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Gene. Vol.149, No.2 (1994), 271-276

Suggested Citation

Catherine Lemaire, Saradee Warit, Sakol Panyim Giant catfish Pangasianodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction. Gene. Vol.149, No.2 (1994), 271-276. doi:10.1016/0378-1119(94)90160-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9504

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Title

Giant catfish Pangasianodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction

Author(s)

Catherine Lemaire
 Saradee Warit
 Sakol Panyim

Other Contributor(s)

Mahidol University

Abstract

cDNA clones encoding giant catfish (Pangasianodon gigas) growth hormone (GH) have been isolated using a polymerase chain reaction (PCR) strategy. Pairwise combinations of degenerate and general primers allowed for the amplification of regions both 3′ and 5′ to the point of entry into the message. The amplified PCR products were cloned and sequenced. The cDNA sequence was found to encode a polypeptide of 200 amino acids (aa), including a putative signal peptide of 22 aa. The 5′ and 3′ untranslated regions of the message are 58 and 515 nucleotides long, respectively. The giant catfish GH displays the highest aa sequence homology with the carp GH, with 80% of sequence identity. Moreover, giant catfish GH has structural features in common with both mammalian and avian GH polypeptides, and also contains the domains of conserved sequence found in other GH. © 1994.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/9504

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Scopus 1991-2000