Publication: Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis
Issued Date
2015-01-01
Resource Type
ISSN
15427536
15427528
15427528
Other identifier(s)
2-s2.0-84946045479
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Crop Improvement. Vol.29, No.6 (2015), 728-746
Suggested Citation
Ratchadaporn Thaikert, Supajit Sraphet, Athipong Boonchanawiwat, Opas Boonseng, Duncan R. Smith, Sittiruk Roytrakul, Kanokporn Triwitayakorn Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis. Journal of Crop Improvement. Vol.29, No.6 (2015), 728-746. doi:10.1080/15427528.2015.1083497 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35279
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Title
Identification of Differentially Expressed Proteins in Cassava Infected with Colletotrichum gloeosporioides f. sp. manihotis
Abstract
Copyright © Taylor & Francis Group, LLC. Cassava anthracnose disease (CAD), caused by Colletotrichum gloeosporioides f. sp. manihotis infection, is a major disease of cassava (Manihot esculenta Crantz). The objective of this study was to identify proteins differentially regulated in resistant (‘Huaybong 60’) and susceptible (‘Hanatee’) cultivars in response to C. gloeosporioides f. sp. manihotis infection. Total proteins were extracted and resolved via 2-dimensional gel electrophoresis. Fourteen differentially expressed proteins were identified in ‘Huaybong 60’, of which eight were up-regulated and seven down-regulated. In ‘Hanatee’, seven proteins were identified, of which one was up-regulated and six were down-regulated. Proteins associated with carbohydrate metabolism were down-regulated, whereas reactive oxygen species-generating and cyanogenic pathways were up-regulated in the resistant cultivar. This study provided new insights into the mechanisms underlying pathogen resistance in cassava and suggested that pathogen resistance might arise from an integrated mechanism that arises from only a few initiating events.
