Publication: Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
Issued Date
2008-01-01
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ISSN
14602377
09538178
09538178
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2-s2.0-37549064726
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Mahidol University
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SCOPUS
Bibliographic Citation
International Immunology. Vol.20, No.1 (2008), 21-29
Suggested Citation
Suttichai Krisanaprakornkit, Pareena Chotjumlong, Prachya Kongtawelert, Vichai Reutrakul Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2. International Immunology. Vol.20, No.1 (2008), 21-29. doi:10.1093/intimm/dxm115 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/19382
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Title
Involvement of phospholipase D in regulating expression of anti-microbial peptide human β-defensin-2
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Abstract
Human β-defensin expression correlates with differentiation in oral epithelium, and calcium ion, an important regulator of epithelial differentiation, plays a critical role in regulation of human β-defensin-2 (hBD-2) mRNA expression. Phospholipase D (PLD) also regulates epithelial differentiation. Therefore, we examined the role of PLD in hBD-2 up-regulation by cell wall extract of Fusobacterium nucleatum and phorbol 12-myristate 13-acetate (PMA), two known hBD-2 activators. We found that hBD-2 mRNA up-regulation in human gingival epithelial cells (HGECs) by these two activators was mediated by PLD activation and blocked by ethanol and 1-butanol, PLD inhibitors. PLD activity was induced by stimulation with these two activators, and phosphatidic acid (PA), a product generated from the PLD enzymatic activity, was detected in stimulated HGECs. Dioctanoyl PA commonly used for PA induced hBD-2 mRNA expression. mRNAs for PLD1α and β splice variants as well as PLD1 protein were constitutively expressed, whereas mRNA and protein for PLD2 were expressed at much lower levels than those for PLD1. Moreover, pre-treatment with (±)-propanolol, an inhibitor of phosphatidic acid phosphohydrolases that are the downstream signaling molecules in the PLD pathway, significantly blocked hBD-2 mRNA induction by PMA in a dose-dependent manner. In conclusion, these findings indicate the involvement of PLD activation in hBD-2 up-regulation in HGECs, which correlates with the state of epithelial differentiation. © The Japanese Society for Immunology. 2007. All rights reserved.