Publication: Identification of a gene cluster responsible for the biosynthesis of cyclic lipopeptide verlamelin
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Issued Date
2014-01-01
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14320614
01757598
01757598
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2-s2.0-84906948513
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Mahidol University
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SCOPUS
Bibliographic Citation
Applied Microbiology and Biotechnology. Vol.98, No.17 (2014), 7501-7510
Suggested Citation
Kei Ichi Ishidoh, Hiroshi Kinoshita, Takuya Nihira Identification of a gene cluster responsible for the biosynthesis of cyclic lipopeptide verlamelin. Applied Microbiology and Biotechnology. Vol.98, No.17 (2014), 7501-7510. doi:10.1007/s00253-014-5803-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33388
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Title
Identification of a gene cluster responsible for the biosynthesis of cyclic lipopeptide verlamelin
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Abstract
Only limited studies are available on the molecular-level biosynthesis of cyclic lipopeptides (cyclic and hybrid molecules consisting of peptide and fatty acid moieties) in filamentous fungi. Here, we identified and characterized biosynthetic genes of the cyclic lipopeptides, known as verlamelins. Only four genes, coding for non-ribosomal peptide synthetase (NRPS), fatty acid hydroxylase, thioesterase, and AMP-dependent ligase, were found to be involved in verlamelin biosynthesis by the analysis of corresponding gene knockouts. Surprisingly, no gene(s) coding for fatty acid synthase or polyketide synthase was present in the cluster, while verlamelin A/B contained a 5-hydroxytetradecanoic acid moiety. Precursor feeding experiment indicated that both fatty acid hydroxylase and thioesterase are involved to supply 5-hydroxytetradecanoic acid. The results suggested that 5-hydroxytetradecanoic acid was supplied from primary metabolism via fatty acid hydroxylase and loaded onto NRPS. Elongation of the peptide and final cyclization were accomplished by NRPS. The knowledge obtained through this study should provide new insight into fungal lipopeptide biosynthesis. © 2014 Springer-Verlag.