Publication: ELISA for immunodiagnosis of human gnathostomiasis
Issued Date
1985-12-01
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ISSN
00383619
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2-s2.0-0022414125
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Mahidol University
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SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.16, No.2 (1985), 274-279
Suggested Citation
P. Suntharasamai, V. Desakorn, S. Migasena, D. Bunnag, T. Harinasuta ELISA for immunodiagnosis of human gnathostomiasis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.16, No.2 (1985), 274-279. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30825
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Title
ELISA for immunodiagnosis of human gnathostomiasis
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Abstract
An ELISA for immunodiagnosis of human gnathostomiasis using a crude water extract of third-stage larvae of G. spinigerum as antigen, and alkaline phosphatase labelled goat antihuman IgG in the indicator system was developed and evaluated. At the titre of 1 : 400 and above positive results were observed in 100% of 4 parasitological confirmed and 10 eosinophilic meningo-encephalitis (EME) typical of gnathostomiasis cases, 56% of 160 cutaneous migratory swelling cases, 33% of 24 cases with EME typical of A. cantonensis infections, 23% of 92 cases with other parasitic infections and 1.5% of blood donors. The overall sensitivity was 59% and specificity 84%. The predictive value was 77%. The results indicated that ELISA is potentially useful for immunodiagnosis of gnathostomiasis but improvement of sensitivity and specificity is needed.