Publication: Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein
Issued Date
2016-09-01
Resource Type
ISSN
14698161
00311820
00311820
Other identifier(s)
2-s2.0-84975134828
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasitology. Vol.143, No.11 (2016), 1369-1381
Suggested Citation
Panat Anuracpreeda, Runglawan Chawengkirttikul, Prasert Sobhon Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein. Parasitology. Vol.143, No.11 (2016), 1369-1381. doi:10.1017/S0031182016001104 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/43387
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection of Fasciola gigantica circulating fatty acid binding protein
Other Contributor(s)
Abstract
© Cambridge University Press 2016. Up to now, parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Hence, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden. In the present study, a monoclonal antibody (MoAb) against recombinant Fasciola gigantica fatty acid binding protein (rFgFABP) has been produced. As well, a reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) has been developed for the detection of circulating FABP in the sera of mice experimentally and cattle naturally infected with F. gigantica. MoAb 3A3 and biotinylated rabbit anti-recombinant FABP antibody were selected due to their high reactivities and specificities. The lower detection limit of sandwich ELISA was 5 pg mL-1, and no cross-reaction with other parasite antigens was observed. This assay could detect F. gigantica infection from day 1 post infection. In experimental mice, the sensitivity, specificity and accuracy of this assay were 93·3, 100 and 98·2%, while in natural cattle they were 96·7, 100 and 99·1%. Hence, this sandwich ELISA method showed high efficiencies and precisions for diagnosis of fasciolosis by F. gigantica.