Publication: Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand
Issued Date
2018-03-01
Resource Type
ISSN
0102695X
Other identifier(s)
2-s2.0-85046708402
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Brazilian Journal of Pharmacognosy. Vol.28, No.2 (2018), 145-150
Suggested Citation
Sumet Kongkiatpaiboon, Savita Chewchinda, Boonyadist Vongsak Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand. Brazilian Journal of Pharmacognosy. Vol.28, No.2 (2018), 145-150. doi:10.1016/j.bjp.2018.03.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47323
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand
Other Contributor(s)
Abstract
© 2018 Sociedade Brasileira de Farmacognosia Pluchea indica (L.) Less., Asteraceae, is a medicinal plant which contains a high amount of phenolic compounds such as caffeoylquinic acid derivatives. The leaves have been traditionally used as a nerve tonic and extensively as herbal tea. This study aimed to develop and validate an HPLC method to quantitatively analyze six caffeoylquinic acid derivatives, viz. 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O caffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid in P. indica leaf extract. HPLC was carried out in a Hypersil BDS C18-column eluted with 0.5% acetic acid in water and methanol using gradient elution with a flow rate of 1 ml/min and detection at 326 nm. The method validation was performed to assure its linearity, precision, accuracy and limits of detection and quantitation. Several extraction techniques including maceration, decoction, digestion, Soxhlet extraction, and ultrasound extraction, were used to extract active constituents. The ultrasound extraction with 50% ethanol yielded the highest concentration of these caffeoylquinic acid derivatives in the P. indica leaf extract. Our developed HPLC method is simple and reliable for a routine analysis of the six caffeoylquinic acids in P. indica leaves and could potentially be applied to be used in commercial herbal products.