Publication: Highly permissive infection of microglial cells by Japanese encephalitis virus: a possible role as a viral reservoir
Issued Date
2010-01-01
Resource Type
ISSN
1769714X
12864579
12864579
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2-s2.0-73149084851
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbes and Infection. Vol.12, No.1 (2010), 37-45
Suggested Citation
Thananya Thongtan, Poonlarp Cheepsunthorn, Voravasa Chaiworakul, Chutima Rattanarungsan, Nitwara Wikan, Duncan R. Smith Highly permissive infection of microglial cells by Japanese encephalitis virus: a possible role as a viral reservoir. Microbes and Infection. Vol.12, No.1 (2010), 37-45. doi:10.1016/j.micinf.2009.09.013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29284
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Title
Highly permissive infection of microglial cells by Japanese encephalitis virus: a possible role as a viral reservoir
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Abstract
Japanese encephalitis virus (JEV), a mosquito-borne Flavivirus, is a major cause of acute encephalitis, and neurons have been proposed to be the principle JEV target cells in the central nervous system. However, clinically, infection with JEV leads to increased levels of cytokines and chemokines in the serum and cerebrospinal fluid (CSF) the levels of which correlate with the mortality rate of patients. This research aimed to study the role of microglial cells in JEV infection. Mouse microglial cells (BV-2) supported the replication of JEV with extracellular production of virus by 10 h post-infection, and virus titer reached a maximum (2.55 × 1010pfu/ml) by day 3 post-infection. While apoptosis was induced in response to virus infection, no alteration in nitric oxide production was observed. Microglial cells remained productively infected with JEV for up to 16 weeks without significant morphological alterations, and the released virions were infectious to mouse neuroblastoma (NA) cells. The high virus production and long persistence of JEV in microglial cells suggests that these cells may serve as viral reservoirs for the infection of neurons in the CNS. © 2009 Elsevier Masson SAS. All rights reserved.