Publication: Proteomic analysis of salinity-stressed Chlamydomonas reinhardtii revealed differential suppression and induction of a large number of important housekeeping proteins
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Issued Date
2012-03-01
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video/youtube
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14322048
00320935
00320935
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2-s2.0-84857631072
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Mahidol University
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SCOPUS
Bibliographic Citation
Planta. Vol.235, No.3 (2012), 649-659
Suggested Citation
Chotika Yokthongwattana, Bancha Mahong, Sittiruk Roytrakul, Narumon Phaonaklop, Jarunya Narangajavana, Kittisak Yokthongwattana Proteomic analysis of salinity-stressed Chlamydomonas reinhardtii revealed differential suppression and induction of a large number of important housekeeping proteins. Planta. Vol.235, No.3 (2012), 649-659. doi:10.1007/s00425-012-1594-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/13494
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Title
Proteomic analysis of salinity-stressed Chlamydomonas reinhardtii revealed differential suppression and induction of a large number of important housekeeping proteins
Abstract
Salinity stress is one of the most common abiotic stresses that hamper plant productivity worldwide. Successful plant adaptations to salt stress require substantial changes in cellular protein expression. In this work, we present a 2-DE-based proteomic analysis of a model unicellular green alga, Chlamydomonas reinhardtii, subjected t o 300 mM NaCl for 2 h. Results showed that, in addition to the protein spots that showed partial up- or down-regulation patterns, a number of proteins were exclusively present in the proteome of the control cells, but were absent from the salinity-stressed samples. Conversely, a large number of proteins exclusively appeared in the proteome of the salinity-stressed samples. Of those exclusive proteins, we could successfully identify, via LC-MS/MS, 18 spots uniquely present in the control cells and 99 spots specific to NaCl-treated cells. Interestingly, among the salt-exclusive protein spots, we identified several important housekeeping proteins like molecular chaperones and proteins of the translation machinery, suggesting that they may originate from post-translational modifications rather than from de novo biosynthesis. The possible role and the salt-specific modification of these proteins by salinity stress are discussed. © 2012 Springer-Verlag.