Publication: The roles of peroxide protective regulons in protecting Xanthomonas campestris pv. Campestris from sodium hypochlorite stress
Issued Date
2015-12-08
Resource Type
ISSN
14803275
00084166
00084166
Other identifier(s)
2-s2.0-84929470552
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Canadian Journal of Microbiology. Vol.61, No.5 (2015), 343-350
Suggested Citation
Nisanart Charoenlap, Phornphan Sornchuer, Anong Piwkam, Kriangsuk Srijaruskul, Skorn Mongkolsuk, Paiboon Vattanaviboon The roles of peroxide protective regulons in protecting Xanthomonas campestris pv. Campestris from sodium hypochlorite stress. Canadian Journal of Microbiology. Vol.61, No.5 (2015), 343-350. doi:10.1139/cjm-2014-0792 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35324
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The roles of peroxide protective regulons in protecting Xanthomonas campestris pv. Campestris from sodium hypochlorite stress
Abstract
The exposure of Xanthomonas campestris pv. campestris to sublethal concentrations of a sodium hypochlorite (NaOCl) solution induced the expression of genes that encode peroxide scavenging enzymes within the OxyR and OhrR regulons. Sensitivity testing in various X. campestris mutants indicated that oxyR, katA, katG, ahpC, and ohr contributed to protection against NaOCl killing. The pretreatment of X. campestris cultures with oxidants, such as hydrogen peroxide (H2O2), t-butyl hydroperoxide, and the superoxide generator menadione, protected the bacteria from lethal concentrations of NaOCl in an OxyR-dependent manner. Treating the bacteria with a low concentration of NaOCl resulted in the adaptive protection from NaOCl killing and also provided cross-protection from H2O2 killing. Taken together, the results suggest that the toxicity of NaOCl is partially mediated by the generation of peroxides and other reactive oxygen species that are removed by primary peroxide scavenging enzymes, such as catalases and AhpC, as a part of an overall strategy that protects the bacteria from the lethal effects of NaOCl.