Publication: Genetic analysis of Cheirostylis species based on microsatellite markers
Issued Date
2015-11-01
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ISSN
1479263X
14792621
14792621
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2-s2.0-84949225988
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Genetic Resources: Characterisation and Utilisation. Vol.13, No.3 (2015), 286-289
Suggested Citation
Supajit Sraphet, Anuwat Saengsri, Duncan R. Smith, Kanokporn Triwitayakorn Genetic analysis of Cheirostylis species based on microsatellite markers. Plant Genetic Resources: Characterisation and Utilisation. Vol.13, No.3 (2015), 286-289. doi:10.1017/S1479262114000914 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35074
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Title
Genetic analysis of Cheirostylis species based on microsatellite markers
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Abstract
© 2014 NIAB. Microsatellite markers specific to Cheirostylis yunnanensis Rolfe were developed using an enriched genomic DNA library technique. The library was constructed using (AG)20 and (CAG)20 oligonucleotide repeats. A total of 48 primer pairs were designed and tested with 48 C. yunnanensis Rolfe samples, resulting in 11 polymorphic loci. The number of alleles per locus ranged from 2 to 12, with an average of six alleles. The observed and expected heterozygosity ranged from 0.0426 to 0.8085 and 0.0421 to 0.9078, respectively. Of the 11 polymorphic loci, three showed a significant deviation from Hardy-Weinberg equilibrium and one exhibited linkage disequilibrium. Cross-species amplification was tested with five samples of Cheirostylis of unknown species resulting in eight loci that could be amplified, with the number of alleles ranging from one to two. The microsatellite markers developed in this study will be useful for the genetic analysis of C. yunnanensis in order to differentiate species as well as to establish a conservation plan for this species.