Indirect haemagglutination test using monoclonal antibody-affinity purified antigens for diagnosis of human paragonimiasis due to Paragonimus heterotremus

Abstract

An indirect haemagglutination test (IHA) using antigens purified by monoclonal antibody-affinity chromatography was developed for the diagnosis of human paragonimiasis caused by Paragonimus heterotremus. Sera from patients with paragonimiasis (n = 30) were evaluated, along with sera from other parasitic infections (n = 92), pulmonary tuberculosis (n = 18) and healthy controls (n = 30). The sensitivity, specificity as well as positive and negative predictive value of the IHA, calculated at the prevalence of disease at 17.6%, were all 100%.