Publication: Antibodies that block virus attachment to vero cells are a major component of the human neutralizing antibody response against dengue virus type 2
1
Issued Date
1995-01-01
Resource Type
ISSN
10969071
01466615
01466615
Other identifier(s)
2-s2.0-0028921203
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Medical Virology. Vol.45, No.4 (1995), 451-461
Suggested Citation
Run‐Tao ‐T He, Songli Wang, Robert Anderson, Bruce L. Innis, Ananda Nisalak, Wipawee Usawattanakul, Siripen Kalayanarooj And Antibodies that block virus attachment to vero cells are a major component of the human neutralizing antibody response against dengue virus type 2. Journal of Medical Virology. Vol.45, No.4 (1995), 451-461. doi:10.1002/jmv.1890450417 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/17329
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Title
Antibodies that block virus attachment to vero cells are a major component of the human neutralizing antibody response against dengue virus type 2
Abstract
Epidemiological data strongly implicate a role for the host humoral immune response in both protection against and exacerbation of dengue virus‐caused disease. In an effort to characterize elements of the normal human immune response against dengue virus we have addressed the issue of antibody‐mediated neutralization of dengue virus. We show here the ability of both mouse monoclonal antibody 3H5 and human anti‐dengue neutralizing sera to block binding of dengue‐2 virus to monkey kidney (Vero) cells. Since Vero cells possess virus receptors but not Fc receptors we conclude that the major effect of host neutralizing antibodies is to block virus attachment to Vero cell dengue virus receptors. Analysis of 61 patient antisera yielded good correlation (Pearson's coefficient = 0.90; P < 0.001) between neutralizing activity and ability to block virus‐cell attachment suggesting that antibody‐mediated neutralization of dengue virus occurs primarily extracellularly and less by a postat‐tachment mechanism as has been described for certain other viruses. © 1995 Wiley‐Liss, inc. Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company