Publication: Construction of a human functional single-chain variable fragment (scFv) antibody recognizing the malaria parasite Plasmodium falciparum
Issued Date
2006-04-01
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08854513
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2-s2.0-33645678287
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Mahidol University
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SCOPUS
Bibliographic Citation
Biotechnology and Applied Biochemistry. Vol.44, No.1 (2006), 55-61
Suggested Citation
Sumet Wajanarogana, Teerawat Prasomrothanakul, Rachanee Udomsangpetch, Sumalee Tungpradabkul Construction of a human functional single-chain variable fragment (scFv) antibody recognizing the malaria parasite Plasmodium falciparum. Biotechnology and Applied Biochemistry. Vol.44, No.1 (2006), 55-61. doi:10.1042/BA20050144 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/23051
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Title
Construction of a human functional single-chain variable fragment (scFv) antibody recognizing the malaria parasite Plasmodium falciparum
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Abstract
Falciparum malaria is one of the most deadly and profound human health problems around the tropical world. Antimalarial drugs are now considered to be a powerful treatment; however, there are drugs currently being used that are resistant to Plasmodium falciparum parasites spreading in different parts of the world. Although the protective immune response against intraerythrocytic stages of the falciparum malaria parasite is still not fully understood, immune antibodies have been shown to be associated with reduced parasite prevalence. Therefore antibodies of the right specificity present in adequate concentrations and affinity are reasonably effective in providing protection. In the present study, VH (variable domain of heavy chain) and VL (variable domain of light chain) were isolated from human blood lymphocytes of P. falciparum in one person who had high serum titre to RESA (ring-infected erythrocyte surface antigen). Equal amounts of VH and VL were assembled together with universal linker (G4S)3 to generate scFvs (single-chain variable fragments). The scFv antibodies were expressed with a phage system for the selection process. Exclusively, an expressed scFv against asynchronous culture of P. falciparum-infected erythrocytes was selected and characterized. Sequence analysis of selected scFv revealed that this clone could be classified into a VH family-derived germline gene (VHI) and VK family segment (V KI). Using an indirect immunofluorescence assay, we could show that soluble expressed scFv reacted with falciparum-infected erythrocytes. The results encourage the further study of scFvs for development as a potential immunotherapeutic agent. © 2006 Portland Press Ltd.